|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the N-terminal region of rat glycine receptor alpha 1 subunit.|
|Storage buffer||Ammonium bicarbonate|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-84890 detects a band at ~48 kDa in rat spinal cord lysates.
Reconstitute with 0.1 ml PBS. For long term storage, the addition of 0.09% sodium azide is recommended. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles.
Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.