Immunohistochemistry was performed on cancer biopsies of deparaffinized Human Prostate and Stomach cancer tissues. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:800 with a Mouse monoclonal antibody recognizing Glyoxylase (Product # MA1-13029) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB and tissues counterstained with hematoxylin. Images are displayed at 40X magnification. Results demonstrate cytoplasmic localization of Glyoxylase.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Mouse / IgG|
|Immunogen||Recombinant human GLOI-GST fusion protein.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-13029X detects glyoxalase-1 in human, monkey, mouse, and rat samples and has been used in immunohistochemistry, immunofluorescence, and western blotting applications.
Glyoxalase-1 catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione and is involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.