Invitrogen

Glypican 3 Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (18) Glypican 3 antibodies

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Datasheet

Questions & Answers

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Glypican 3 Antibody in Western Blot (WB) — Western blot was performed using Glypican 3 Polyclonal Antibody (Product # PA5-47256) and a 66 kDa band corresponding to GPC3 was observed only in Hep G2. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3) and T-47D (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Rabbit anti-Sheep IgG (H+L), Secondary Antibody, HRP (Product # 61-8620, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Glypican 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Glypican 3 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Sheep Anti-human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47256) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB (brown and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Glypican 3 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models tested owing to their inherent genetic constitution. Relative expression of GPC3 was observed in Hep G2 in comparison to HeLa, MCF7 and T-47D using Glypican 3 polyclonal antibody (Product # PA5-47256) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL

Flow Cytometry (Flow)

2.5 µg/1x10^6 cells

Product Specifications

Species Reactivity

Human

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glypican 3 Gln25-Val558

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2608607

Product Specific Information

In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Glypican 3 is observed and less than 5% cross-reactivity with recombinant human (rh) Glypican 2, rhGlypican 5, and rhGlypican 6 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: glypican proteoglycan 3; Glypican-3; Glypican-3 alpha subunit; Glypican-3 beta subunit; GTR2-2; GTR22; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; secreted glypican-3

Gene Aliases: DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS; SGBS1

UniProt ID: (Human) P51654

Entrez Gene ID: (Human) 2719

Function(s)