Invitrogen

Glypican 3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Reference

View all (49) Glypican 3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (6)
Advanced Verification (1)

Glypican 3 Antibody in Western Blot (WB)

FIGURE: 1 / 7

Glypican 3 Antibody (PA5-47256) in WB

Western blot was performed using Glypican 3 Polyclonal Antibody (Product # PA5-47256) and a 66 kDa band corresponding to GPC3 was observed only in Hep G2. Membrane enriched extracts (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), MCF7 (Lane 3) and T-47D (Lane 4) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 ... View More

Product Image Gallery

Glypican 3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Glypican 3 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in Glypican 3 polyclonal antibody (Product # PA5-47256) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Glypican 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Glypican 3 in immersion fixed paraffin-embedded sections of human liver cancer tissue. Samples were incubated in Glypican 3 polyclonal antibody (Product # PA5-47256) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Glypican 3 Antibody in ELISA (ELISA) — ELISA of Glypican 3 in recombinant Glypican 3 protein. Samples were incubated in Glypican 3 polyclonal antibody (Product # PA5-47256). Protein was serially diluted 2-fold. Antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP followed by Substrate Solution and stopping the enzymatic reaction with Stop Solution.

Glypican 3 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models tested owing to their inherent genetic constitution. Relative expression of GPC3 was observed in Hep G2 in comparison to HeLa, MCF7 and T-47D using Glypican 3 polyclonal antibody (Product # PA5-47256) in Western Blot. {RE}

Product Details

PA5-47256

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5-15 µg/mL

Immunocytochemistry (ICC/IF)

1-15 µg/mL

Flow Cytometry (Flow)

2.5 µg per million cells

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Glypican 3 Gln25-Val558

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2608607

Product Specific Information

In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Glypican 3 is observed and less than 5% cross-reactivity with recombinant human (rh) Glypican 2, rhGlypican 5, and rhGlypican 6 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Target Information

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transferrin Receptor Is a Specific Ferroptosis Marker.

Cell reports

Feng H,Schorpp K,Jin J,Yozwiak CE,Hoffstrom BG,Decker AM,Rajbhandari P,Stokes ME,Bender HG,Csuka JM,Upadhyayula PS,Canoll P,Uchida K,Soni RK,Hadian K,Stockwell BR

PA5-47256 was used in Immunohistochemistry-immunofluorescence to find that anti-TfR1 and anti-malondialdehyde adduct antibodies are effective at staining ferroptotic tumor cells in multiple cell culture and tissue contexts.

Tue Mar 10 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: glypican proteoglycan 3; Glypican-3; Glypican-3 alpha subunit; Glypican-3 beta subunit; GTR2-2; GTR22; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; secreted glypican-3

Gene Aliases: DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS; SGBS1

UniProt ID: (Human) P51654

Entrez Gene ID: (Human) 2719

Function(s)

retinoid metabolic process branching involved in ureteric bud morphogenesis glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process anatomical structure morphogenesis anterior/posterior axis specification body morphogenesis negative regulation of peptidase activity glycosaminoglycan metabolic process bone mineralization osteoclast differentiation lung development positive regulation of BMP signaling pathway embryonic hindlimb morphogenesis positive regulation of protein catabolic process positive regulation of endocytosis negative regulation of smoothened signaling pathway positive regulation of smoothened signaling pathway negative regulation of growth positive regulation of glucose import negative regulation of epithelial cell proliferation coronary vasculature development mesenchymal cell proliferation involved in ureteric bud development mesonephric duct morphogenesis cell proliferation involved in metanephros development negative regulation of canonical Wnt signaling pathway positive regulation of Wnt signaling pathway, planar cell polarity pathway

Process(es)

protein binding heparan sulfate proteoglycan binding peptidyl-dipeptidase inhibitor activity