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Invitrogen
Glypican 3 Monoclonal Antibody (GT2473)
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FIGURE: 1 / 8
Glypican 3 Antibody (MA5-31552) in ICC/IF
Glypican 3 Monoclonal Antibody (GT2473) detects Glypican-3 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glypican-3 protein stained by Glypican 3 Monoclonal Antibody (GT2473) (Product # MA5-31552) diluted at 1:250. Blue: Hoechst 33342 staining.
Product Details
MA5-31552
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GT2473
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2787180
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Chimpanzee (96%), Bovine (81%).
Positive Control: HepG2, HepG2 (24 µg/mL Tunicamycin treatment for 16 hr).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glypican proteoglycan 3; Glypican-3; GTR2-2; GTR22; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; secreted glypican-3
Gene Aliases: DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS; SGBS1
UniProt ID: (Human) P51654
Entrez Gene ID: (Human) 2719
retinoid metabolic process
branching involved in ureteric bud morphogenesis
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
anatomical structure morphogenesis
anterior/posterior axis specification
body morphogenesis
negative regulation of peptidase activity
glycosaminoglycan metabolic process
bone mineralization
osteoclast differentiation
lung development
positive regulation of BMP signaling pathway
embryonic hindlimb morphogenesis
positive regulation of protein catabolic process
positive regulation of endocytosis
negative regulation of smoothened signaling pathway
positive regulation of smoothened signaling pathway
negative regulation of growth
positive regulation of glucose import
negative regulation of epithelial cell proliferation
coronary vasculature development
mesenchymal cell proliferation involved in ureteric bud development
mesonephric duct morphogenesis
cell proliferation involved in metanephros development
negative regulation of canonical Wnt signaling pathway
positive regulation of Wnt signaling pathway, planar cell polarity pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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