Invitrogen

Glypican 3 Monoclonal Antibody (GT2473)

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Glypican 3 Antibody in Western Blot (WB) — Western blot analysis of Glypican 3 in whole cell extract using Glypican 3 monoclonal antibody (Product # MA5-31552) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Glypican 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Glypican 3 in paraffin-embedded human hepatocellular carcinoma using Glypican 3 monoclonal antibody (Product # MA5-31552) at a dilution of 1:100.

Glypican 3 Antibody in Immunofluorescence (IF) — Immunocytochemistry analysis of Glypican 3 in 4% paraformaldehyde-fixed HepG2 cells using Glypican 3 monoclonal antibody (Product # MA5-31552) at a dilution of 1:250. Sample was then incubated with Hoechst secondary antibody.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT2473

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.19 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2787180

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Chimpanzee (96%), Bovine (81%).

Positive Control: HepG2, HepG2 (24 µg/ml Tunicamycin treatment for 16 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: glypican proteoglycan 3; Glypican-3; Glypican-3 alpha subunit; Glypican-3 beta subunit; GTR2-2; GTR22; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; secreted glypican-3

Gene Aliases: DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS; SGBS1

UniProt ID: (Human) P51654

Entrez Gene ID: (Human) 2719

Function(s)