Invitrogen

Glypican 3 Monoclonal Antibody (GT2473)

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Glypican 3 Antibody in Immunofluorescence (IF) — Immunocytochemistry analysis of Glypican 3 in 4% paraformaldehyde-fixed HepG2 cells using Glypican 3 monoclonal antibody (Product # MA5-31552) at a dilution of 1:250. Sample was then incubated with Hoechst secondary antibody.

Glypican 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Glypican 3 in paraffin-embedded human hepatocellular carcinoma using Glypican 3 monoclonal antibody (Product # MA5-31552) at a dilution of 1:100.

Glypican 3 Antibody in Western Blot (WB) — Western blot analysis of Glypican 3 in whole cell extract using Glypican 3 monoclonal antibody (Product # MA5-31552) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT2473

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Glypican-3.

Conjugate

Unconjugated

Form

Liquid

Concentration

2.19 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Keep as concentrated solution.

Positive Control: HepG2, HepG2 (24 µg/ml Tunicamycin treatment for 16 hr).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DGSX antibody; glypican 3 antibody; glypican proteoglycan 3; Glypican-3; Glypican-3 alpha subunit; Glypican-3 antibody; Glypican-3 antibody [GT2473] antibody; Glypican-3 beta subunit; GPC3 antibody; GTR2-2; GTR22 antibody; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; MXR7 antibody; OCI5 antibody; SDYS antibody; secreted glypican-3; SGB antibody; SGBS antibody; SGBS1 antibody

Gene Aliases: DGSX; GPC3; GTR2-2; MXR7; OCI-5; OCI5; SDYS; SGB; SGBS; SGBS1

UniProt ID: (Human) P51654

Entrez Gene ID: (Human) 2719

Function(s)

retinoid metabolic process branching involved in ureteric bud morphogenesis glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process anatomical structure morphogenesis anterior/posterior axis specification body morphogenesis negative regulation of peptidase activity glycosaminoglycan metabolic process bone mineralization osteoclast differentiation lung development positive regulation of BMP signaling pathway embryonic hindlimb morphogenesis positive regulation of protein catabolic process positive regulation of endocytosis negative regulation of smoothened signaling pathway positive regulation of smoothened signaling pathway negative regulation of growth positive regulation of glucose import negative regulation of epithelial cell proliferation coronary vasculature development mesenchymal cell proliferation involved in ureteric bud development mesonephric duct morphogenesis cell proliferation involved in metanephros development negative regulation of canonical Wnt signaling pathway positive regulation of Wnt signaling pathway, planar cell polarity pathway

Process(es)

protein binding heparan sulfate proteoglycan binding peptidyl-dipeptidase inhibitor activity

Glypican 3 Monoclonal Antibody (GT2473)

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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