Invitrogen

Glypican 4 Polyclonal Antibody

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Glypican 4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Glypican 4 in HepG2 cells using a Glypican 4 polyclonal antibody (Product # PA5-97801) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.

Glypican 4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Glypican 4 in paraffin embedded human pancreatic tissue using a Glypican 4 polyclonal antibody (Product # PA5-97801) at a dilution of 1:100.

Glypican 4 Antibody in Western Blot (WB) — Western Blot analysis of Glypican 4 using a Glypican 4 Polyclonal antibody (Product # PA5-97801) at a concentration of 8 µg/mL. Positive WB detected in Mouse brain tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:10,000 dilution. Observed band size: 63 kDa.

Product Details

PA5-97801

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
ELISA (ELISA)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant Human Glypican-4 protein (19-210AA) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	4 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.03% Proclin 300
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2812416

Target Information

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes.

eLife

Ghoroghi S,Mary B,Larnicol A,Asokan N,Klein A,Osmani N,Busnelli I,Delalande F,Paul N,Halary S,Gros F,Fouillen L,Haeberle AM,Royer C,Spiegelhalter C,André-Grégoire G,Mittelheisser V,Detappe A,Murphy K,Timpson P,Carapito R,Blot-Chabaud M,Gavard J,Carapito C,Vitale N,Lefebvre O,Goetz JG,Hyenne V