|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-120 detects gonadotropin releasing hormone (GnRH) in mouse brain.
PA1-120 has been successfully used in immunofluorescence and immunohistochemistry procedures. Immunofluorescent staining of GnRH in mouse embryo brain tissue using PA1-120 results in staining of neurons in the rostral forebrain.
In immunohistochemical procedures, 4% paraformaldehyde is recommended as a fixative.
The PA1-120 immunogen is a synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH. This peptide (Cat. # PEP-168) is available for use in neutralization and control experiments.
Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.
Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Neurokinin B is critical for normal timing of sexual maturation but dispensable for adult reproductive function in female mice.
PA1-120 was used in immunohistochemistry - paraffin section to use Tac2 deficient mice to study neurokinin B-signaling and sexual maturation
|True C,Nasrin Alam S,Cox K,Chan YM,Seminara SB||Endocrinology (156:1386)||2015|
Atrazine inhibits pulsatile gonadotropin-releasing hormone (GnRH) release without altering GnRH messenger RNA or protein levels in the female rat.
PA1-120 was used in immunohistochemistry to study the ability of atrazine to inhibit pulsilate release of gonadotrophin releasing hormone in female rats whilst expression of homone mRNA and protein remain unchanged
|Foradori CD,Zimmerman AD,Hinds LR,Zuloaga KL,Breckenridge CB,Handa RJ||Biology of reproduction (88:null)||2013|
Detection and localization of an estrogen receptor beta splice variant protein (ERbeta2) in the adult female rat forebrain and midbrain regions.
PA1-120 was used in immunohistochemistry to investigate the expression and distribution of an estrogen receptor beta 2 isoform in adult female rat brain
|Chung WC,Pak TR,Suzuki S,Pouliot WA,Andersen ME,Handa RJ||The Journal of comparative neurology (505:249)||2007|