|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-122 detects gonadotropin releasing hormone (GnRH) in mouse brain.
PA1-122 has been successfully used in immunohistochemistry procedures. Immunohistochemical staining of GnRH in newborn mouse brain tissue using PA1-122 results in neuronal staining.
In immunohistochemical procedures, 4% paraformaldehyde or 2% acrolein is recommended as a fixative.
The PA1-122 immunogen is a synthetic peptide corresponding to residues pyroE(22) H W S Y G L R P G-NH2(32) of mouse GnRH. This sequence is completely conserved in rat and tree shrew and is 78% conserved in zebrafish. The PA1-122 immunizing peptide (Cat. # PEP-168) is available for use in neutralization and control experiments.
Gonadotropin releasing hormone (GnRH), also known as luteinizing hormone releasing hormone (LHRH), is a key molecule in the regulation of reproduction in vertebrates. GnRH, a decapeptide, is produced by neurons in the medial basal hypothalamus (MBH) and secreted in a pulsatile manner into the cardiovascular system. The frequency and amplitude of GnRH pulses determine secretion of follicle stimulating hormone (FSH) and luteinizing hormone (LH) from the pituitary. Higher frequencies (greater than one pulse per hour) stimulate LH secretion while lower frequencies stimulate FSH secretion. The generation of GnRH pulses is effected by numerous stimuli, such as neural, hormonal and environmental. Therefore, behavioral and physiological conditions such as sleep, exercise, and stress can affect the GnRH pulses and cause a disruption of the normal cycle.
Recent studies show that GnRH also has a role in mediating cancer. GnRH has been shown to inhibit the growth of human uterine leiomyloma cells by suppressing proliferation and inducing apoptosis. GnRH analogs have been used to treat a wide variety of reproductive cancers, although the side effects of using such compounds are often quite severe.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
KISS1R signals independently of G¿q/11 and triggers LH secretion via the ß-arrestin pathway in the male mouse.
PA1-122 was used in immunohistochemistry - free floating to test if KISSR regulates GnRH secretion via both the Gαq/- and β-arrestin-coupled pathways.
|Ahow M,Min L,Pampillo M,Nash C,Wen J,Soltis K,Carroll RS,Glidewell-Kenney CA,Mellon PL,Bhattacharya M,Tobet SA,Kaiser UB,Babwah AV||Endocrinology (155:4433)||2014|
The neuroregenerative mechanism mediated by the Hsp90-binding immunophilin FKBP52 resembles the early steps of neuronal differentiation.
PA1-122 was used in immunohistochemistry to investigate the mechanism for the neuroregeneration effect of immunosuppressive macrolide FK506
|Quintá HR,Galigniana MD||British journal of pharmacology (166:637)||2012|
Deletion of Otx2 in GnRH neurons results in a mouse model of hypogonadotropic hypogonadism.
PA1-122 was used in immunohistochemistry to investigate the effect of Otx2 mutation in GnRH neurons
|Diaczok D,DiVall S,Matsuo I,Wondisford FE,Wolfe AM,Radovick S||Molecular endocrinology (Baltimore, Md.) (25:833)||2011|
The gonadotropin-releasing hormone cell-specific element is required for normal puberty and estrous cyclicity.
PA1-122 was used in immunohistochemistry to study the promoter element in gonadotropin-releasing hormone gene and its role in puberty and estrous cyclicity
|Novaira HJ,Yates M,Diaczok D,Kim H,Wolfe A,Radovick S||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:3336)||2011|
Divergent roles of growth factors in the GnRH regulation of puberty in mice.
PA1-122 was used in immunohistochemistry to investigate the roles of growth factors in puberty initiation and progression in mice
|Divall SA,Williams TR,Carver SE,Koch L,Brüning JC,Kahn CR,Wondisford F,Radovick S,Wolfe A||The Journal of clinical investigation (120:2900)||2010|