|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Storage buffer||PBS with 1.5% BSA|
|Storage Conditions||4° C|
|Cross Adsorption||Against human, rabbit, rat, horse, guinea pig, hamster, chicken and mouse IgG|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1/5,000 - 1/100,000|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:5000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:5000|
|Western Blot (WB)||1/5,000 - 1/100,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Store at 4°C prior to reconstitution. After reconstitution store at -20°C. For long term storage the addition of 0.01% thiomersal is recommended.
Reconstitute with 0.5 ml of distilled water.
Thermo Scientific Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A failure in energy metabolism and antioxidant uptake precede symptoms of Huntington's disease in mice.
PA1-86326 was used in western blot to study the role of defective neuronal ascorbic acid transport in the development of Huntington's disease
|Acuña AI,Esparza M,Kramm C,Beltrán FA,Parra AV,Cepeda C,Toro CA,Vidal RL,Hetz C,Concha II,Brauchi S,Levine MS,Castro MA||Nature communications (4:null)||2013|
|Not Applicable||Not Cited||
Dengue 2 infection of HepG2 liver cells results in endoplasmic reticulum stress and induction of multiple pathways of cell death.
PA1-86326 was used in western blot to study the induction of ER stress and multiple cell death pathways following Dengue virus-2 infection of HepG2 hepatoma cells
|Thepparit C,Khakpoor A,Khongwichit S,Wikan N,Fongsaran C,Chingsuwanrote P,Panraksa P,Smith DR||BMC research notes (6:null)||2013|