Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Goat|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against human, mouse and rabbit serum proteins|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:20,000 - 1:400,000|
|Flow Cytometry (Flow)||1:200 - 1:1,000|
|Immunocytochemistry (ICC)||1:500 - 1:5,000|
|Immunofluorescence (IF)||1:500 - 1:5,000|
|Immunohistochemistry (IHC)||1:500 - 1:5,000|
|Immunoprecipitation (IP)||1:500 - 1:5,000|
|Western Blot (WB)||1:20,000 - 1:400,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31730 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Product # 31730 reacts with the heavy chains of goat IgG and with the light chains common to most goat immunoglobulins, but does not react against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, mouse, and rabbit serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Reconstitute with 1.0 ml of distilled water (1.5 mg/ml after restoration, lot-dependent).
Country of Origin: USA
Thermo Scientific Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.