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ELISA was performed to detect Goat IgG (GT003, coating conc. 0.2 ug/ml) with serial dilutions (3 fold starting at 1ug/ml) of Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, Alkaline Phosphatase conjugate (A27021). A non-linear regression analysis was performed and EC50 determined to be 0.04ug/ml.
|Tested species reactivity||Goat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant full-length protein|
|Purification||Size-exclusion - Dialysis|
|Storage buffer||PBS with 50% glycerol, 1mM MgCl2, 1mM ZnCl2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.01-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed.
Superclonal™ Secondary Antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced sensitivity and specificity to the target species IgG. These recombinant secondary antibodies show exceptional performance in common immunoassay applications, with very little cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin ensures lot-to-lot consistency, hence eliminating the need to optimize each lot in relevant assays. Our Superclonal™ secondary antibodies allow scientists to achieve the accurate results they need, without the cross-reactivity concerns that can impact some traditional polyclonal secondary antibodies. Superclonal™ secondaries are available with Alexa Fluor conjugates, as well as HRP, AP and biotin. The Molecular Probes Alexa Fluor dyes have set the industry standard for brightness and photostability for several years. Molecular Probes Alexa Fluor secondary antibodies are trusted, having been used in over 30,000 peer reviewed papers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.