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|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 15mg/ml BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:500-1:5000|
|Western Blot (WB)||1:5,000 - 1:200,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
This antibody has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.
Antibody Specificity: The antibody reacts with the heavy chains of goat IgG and with the light chains common to most goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 ml distilled water (0.8 mg/ml after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day.
To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
Thermo Scientific Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
DNA damage induces GDNF secretion in the tumor microenvironment with paracrine effects promoting prostate cancer treatment resistance.
31402 was used in western blot to study how glial cell line-derived neurotrophic factor promotes prostate cancer growth.
|Huber RM,Lucas JM,Gomez-Sarosi LA,Coleman I,Zhao S,Coleman R,Nelson PS||Oncotarget (6:2134)||2015|
The beneficial effect of a prolyl oligopeptidase inhibitor, KYP-2047, on alpha-synuclein clearance and autophagy in A30P transgenic mouse.
31402 was used in western blot to study the role of increased autophagy in the mechanism by which a prolyl oligopeptidase inhibitor decreases accumulation of aggregated alpha-synuclein
|Savolainen MH,Richie CT,Harvey BK,Männistö PT,Maguire-Zeiss KA,Myöhänen TT||Neurobiology of disease (68:1)||2014|
AXL receptor tyrosine kinase is increased in patients with heart failure.
31402 was used in western blot to study the elevated myocardial expression and serum levels of the AXL receptor tyrosine kinase in patients with heart failure
|Batlle M,Recarte-Pelz P,Roig E,Castel MA,Cardona M,Farrero M,Ortiz JT,Campos B,Pulgarín MJ,Ramírez J,Pérez-Villa F,García de Frutos P||International journal of cardiology (173:402)||2014|
|Not Applicable||Not Cited||
A newly discovered LGI1 mutation in Korean family with autosomal dominant lateral temporal lobe epilepsy.
31402 was used in western blot to report on a Korean family with autosomal dominant lateral temporal lobe epilepsy who carry a novel LGI1 mutation
|Lee MK,Kim SW,Lee JH,Cho YJ,Kim DE,Lee BI,Kim HM,Lee MG,Heo K||Seizure (23:69)||2014|
Role of donor and host cells in muscle-derived stem cell-mediated bone repair: differentiation vs. paracrine effects.
31402 was used in immunocytochemistry and western blot to study the contribution of paracrine effects to the repair of bone by muscle-derived stem cells
|Gao X,Usas A,Proto JD,Lu A,Cummins JH,Proctor A,Chen CW,Huard J||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (28:3792)||2014|
|Not Applicable||0.8 mg/ml||
Development of an enzyme-linked immunosorbent assay based on the murine leukemia virus p30 capsid protein.
31402 was used in ELISA to develop an elisa assay for the quantitation of the MuLV p30 capsid protein
|Wu DT,Aiyer S,Villanueva RA,Roth MJ||Journal of virological methods (193:332)||2013|