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|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against rabbit, rat, mouse and human IgG|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
To minimize cross-reactivity, these donkey anti-goat IgG whole antibodies have been cross-adsorbed against rabbit, rat, mouse, and human IgG. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.
Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.
Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Basal forebrain circuit for sleep-wake control.
A-11055 was used in immunohistochemistry - frozen section to study the basal forebrain circuit.
|Xu M,Chung S,Zhang S,Zhong P,Ma C,Chang WC,Weissbourd B,Sakai N,Luo L,Nishino S,Dan Y||Nature neuroscience (18:1641)||2015|
Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2.
A-11055 was used in immunohistochemistry - frozen section to characterize the sites of PGD2 formation and of its receptors in guinea pig bladder
|Guan NN,Svennersten K,de Verdier PJ,Wiklund NP,Gustafsson LE||British journal of pharmacology (172:4024)||2015|
Ventral pallidal projections to mediodorsal thalamus and ventral tegmental area play distinct roles in outcome-specific Pavlovian-instrumental transfer.
A-11055 was used in immunohistochemistry - frozen section to study the Pavlovian-instrumental transfer effect of the rostral medial ventral pallidum region innervated by the nucleus accumbens shell
|Leung BK,Balleine BW||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:4953)||2015|
|Not Applicable||Not Cited||
Osteoclasts and their precursors are present in the induced-membrane during bone reconstruction using the Masquelet technique.
A-11055 was used in immunohistochemistry (frozen) to assess the Masquelet long bone reconstruction technique in a rat model.
|Gouron R,Petit L,Boudot C,Six I,Brazier M,Kamel S,Mentaverri R||Journal of tissue engineering and regenerative medicine (null:null)||2014|
|Not Applicable||Not Cited||
Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.
A-11055 was used in flow cytometry to investigate how heme regulates Spic in macrophages.
|Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM||Cell (156:1223)||2014|
|Not Applicable||Not Cited||Targeting organic anion transporter 3 with probenecid as a novel anti-influenza a virus strategy.||Perwitasari O,Yan X,Johnson S,White C,Brooks P,Tompkins SM,Tripp RA||Antimicrobial agents and chemotherapy (57:475)||2013|
|Not Applicable||Not Cited||The imprinted NPAP1/C15orf2 gene in the Prader-Willi syndrome region encodes a nuclear pore complex associated protein.||Neumann LC,Markaki Y,Mladenov E,Hoffmann D,Buiting K,Horsthemke B||Human molecular genetics (21:4038)||2012|
|Not Applicable||Not Cited||Micron-scale spatially patterned, covalently immobilized vascular endothelial growth factor on hydrogels accelerates endothelial tubulogenesis and increases cellular angiogenic responses.||Leslie-Barbick JE,Shen C,Chen C,West JL||Tissue engineering. Part A (17:221)||2011|
|Not Applicable||Not Cited||SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages.||Berger SB,Romero X,Ma C,Wang G,Faubion WA,Liao G,Compeer E,Keszei M,Rameh L,Wang N,Boes M,Regueiro JR,Reinecker HC,Terhorst C||Nature immunology (11:920)||2010|
|Not Applicable||Not Cited||Respiratory syncytial virus limits alpha subunit of eukaryotic translation initiation factor 2 (eIF2alpha) phosphorylation to maintain translation and viral replication.||Groskreutz DJ,Babor EC,Monick MM,Varga SM,Hunninghake GW||The Journal of biological chemistry (285:24023)||2010|
|Not Applicable||Not Cited||Visualization and identification of IL-7 producing cells in reporter mice.||Mazzucchelli RI,Warming S,Lawrence SM,Ishii M,Abshari M,Washington AV,Feigenbaum L,Warner AC,Sims DJ,Li WQ,Hixon JA,Gray DH,Rich BE,Morrow M,Anver MR,Cherry J,Naf D,Sternberg LR,McVicar DW,Farr AG,Germain RN,Rogers K,Jenkins NA,Copeland NG,Durum SK||PloS one (4:null)||2009|
|Not Applicable||Not Cited||Dynamin 2 mutations associated with human diseases impair clathrin-mediated receptor endocytosis.||Bitoun M,Durieux AC,Prudhon B,Bevilacqua JA,Herledan A,Sakanyan V,Urtizberea A,Cartier L,Romero NB,Guicheney P||Human mutation (30:1419)||2009|
|Not Applicable||Not Cited||Protective role of endogenous gangliosides for lysosomal pathology in a cellular model of synucleinopathies.||Wei J,Fujita M,Nakai M,Waragai M,Sekigawa A,Sugama S,Takenouchi T,Masliah E,Hashimoto M||The American journal of pathology (174:1891)||2009|
|Not Applicable||Not Cited||Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.||Schermelleh L,Carlton PM,Haase S,Shao L,Winoto L,Kner P,Burke B,Cardoso MC,Agard DA,Gustafsson MG,Leonhardt H,Sedat JW||Science (New York, N.Y.) (320:1332)||2008|
|Not Applicable||Not Cited||Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.||Estes JD,Gordon SN,Zeng M,Chahroudi AM,Dunham RM,Staprans SI,Reilly CS,Silvestri G,Haase AT||Journal of immunology (Baltimore, Md. : 1950) (180:6798)||2008|
|Not Applicable||Not Cited||Helicobacter pylori evolution during progression from chronic atrophic gastritis to gastric cancer and its impact on gastric stem cells.||Giannakis M,Chen SL,Karam SM,Engstrand L,Gordon JI||Proceedings of the National Academy of Sciences of the United States of America (105:4358)||2008|
|Not Applicable||Not Cited||Induction of pluripotent stem cells from adult human fibroblasts by defined factors.||Takahashi K,Tanabe K,Ohnuki M,Narita M,Ichisaka T,Tomoda K,Yamanaka S||Cell (131:861)||2007|
|Not Applicable||Not Cited||Upregulation of tPA/plasminogen proteolytic system in the periphery of amyloid deposits in the Tg2576 mouse model of Alzheimer's disease.||Lee JY,Kweon HS,Cho E,Lee JY,Byun HR,Kim DH,Kim YH,Han PL,Koh JY||Neuroscience letters (423:82)||2007|
|Not Applicable||Not Cited||Dlx transcription factors promote migration through repression of axon and dendrite growth.||Cobos I,Borello U,Rubenstein JL||Neuron (54:873)||2007|
|Not Applicable||Not Cited||Immunosuppressive and anti-angiogenic sphingosine 1-phosphate receptor-1 agonists induce ubiquitinylation and proteasomal degradation of the receptor.||Oo ML,Thangada S,Wu MT,Liu CH,Macdonald TL,Lynch KR,Lin CY,Hla T||The Journal of biological chemistry (282:9082)||2007|
|Not Applicable||Not Cited||Post-translational arginylation of calreticulin: a new isospecies of calreticulin component of stress granules.||Decca MB,Carpio MA,Bosc C,Galiano MR,Job D,Andrieux A,Hallak ME||The Journal of biological chemistry (282:8237)||2007|
|Not Applicable||Not Cited||DeltaF508 mutation results in impaired gastric acid secretion.||Sidani SM,Kirchhoff P,Socrates T,Stelter L,Ferreira E,Caputo C,Roberts KE,Bell RL,Egan ME,Geibel JP||The Journal of biological chemistry (282:6068)||2007|
|Not Applicable||Not Cited||P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.||Domercq M,Brambilla L,Pilati E,Marchaland J,Volterra A,Bezzi P||The Journal of biological chemistry (281:30684)||2006|
|Not Applicable||Not Cited||Cyclin I protects podocytes from apoptosis.||Griffin SV,Olivier JP,Pippin JW,Roberts JM,Shankland SJ||The Journal of biological chemistry (281:28048)||2006|
|Not Applicable||Not Cited||Molecular dissection of the interaction between amyloid precursor protein and its neuronal trafficking receptor SorLA/LR11.||Andersen OM,Schmidt V,Spoelgen R,Gliemann J,Behlke J,Galatis D,McKinstry WJ,Parker MW,Masters CL,Hyman BT,Cappai R,Willnow TE||Biochemistry (45:2618)||2006|
|Not Applicable||Not Cited||In vivo identification of ribonucleoprotein-RNA interactions.||Zielinski J,Kilk K,Peritz T,Kannanayakal T,Miyashiro KY,Eiríksdóttir E,Jochems J,Langel U,Eberwine J||Proceedings of the National Academy of Sciences of the United States of America (103:1557)||2006|
|Not Applicable||Not Cited||Evidence that stem cells reside in the adult Drosophila midgut epithelium.||Micchelli CA,Perrimon N||Nature (439:475)||2006|
|Not Applicable||Not Cited||Targeted ablation of plectin isoform 1 uncovers role of cytolinker proteins in leukocyte recruitment.||Abrahamsberg C,Fuchs P,Osmanagic-Myers S,Fischer I,Propst F,Elbe-Bürger A,Wiche G||Proceedings of the National Academy of Sciences of the United States of America (102:18449)||2005|
|Not Applicable||Not Cited||Mouse and human resistins impair glucose transport in primary mouse cardiomyocytes, and oligomerization is required for this biological action.||Graveleau C,Zaha VG,Mohajer A,Banerjee RR,Dudley-Rucker N,Steppan CM,Rajala MW,Scherer PE,Ahima RS,Lazar MA,Abel ED||The Journal of biological chemistry (280:31679)||2005|
|Not Applicable||Not Cited||LPS receptor (CD14): a receptor for phagocytosis of Alzheimer's amyloid peptide.||Liu Y,Walter S,Stagi M,Cherny D,Letiembre M,Schulz-Schaeffer W,Heine H,Penke B,Neumann H,Fassbender K||Brain : a journal of neurology (128:1778)||2005|
|Not Applicable||Not Cited||A rapid enzymatic method for the isolation of defined kidney tubule fragments from mouse.||Wagner CA,Lükewille U,Valles P,Breton S,Brown D,Giebisch GH,Geibel JP||Pflu¿gers Archiv : European journal of physiology (446:623)||2003|
|Not Applicable||Not Cited||Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition.||Buffelli M,Burgess RW,Feng G,Lobe CG,Lichtman JW,Sanes JR||Nature (424:430)||2003|
|Not Applicable||Not Cited||Identification of an organelle-specific myosin V receptor.||Ishikawa K,Catlett NL,Novak JL,Tang F,Nau JJ,Weisman LS||The Journal of cell biology (160:887)||2003|
|Not Applicable||Not Cited||Screening for gene function in chicken embryo using RNAi and electroporation.||Pekarik V,Bourikas D,Miglino N,Joset P,Preiswerk S,Stoeckli ET||Nature biotechnology (21:93)||2003|
|Not Applicable||Not Cited||MBD3 and HDAC1, two components of the NuRD complex, are localized at Aurora-A-positive centrosomes in M phase.||Sakai H,Urano T,Ookata K,Kim MH,Hirai Y,Saito M,Nojima Y,Ishikawa F||The Journal of biological chemistry (277:48714)||2002|
|Not Applicable||Not Cited||Nonpalmitoylated human asialoglycoprotein receptors recycle constitutively but are defective in coated pit-mediated endocytosis, dissociation, and delivery of ligand to lysosomes.||Yik JH,Saxena A,Weigel JA,Weigel PH||The Journal of biological chemistry (277:40844)||2002|
|Not Applicable||Not Cited||Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.||Hirsch JD,Eslamizar L,Filanoski BJ,Malekzadeh N,Haugland RP,Beechem JM,Haugland RP||Analytical biochemistry (308:343)||2002|
|Not Applicable||Not Cited||Role of recycling endosomes and lysosomes in dynein-dependent entry of canine parvovirus.||Suikkanen S,Sääjärvi K,Hirsimäki J,Välilehto O,Reunanen H,Vihinen-Ranta M,Vuento M||Journal of virology (76:4401)||2002|
|Not Applicable||Not Cited||An evolutionary conserved region in the vasa 3'UTR targets RNA translation to the germ cells in the zebrafish.||Knaut H,Steinbeisser H,Schwarz H,Nüsslein-Volhard C||Current biology : CB (12:454)||2002|
|Not Applicable||Not Cited||Cytoplasmic tail-dependent internalization of membrane-type 1 matrix metalloproteinase is important for its invasion-promoting activity.||Uekita T,Itoh Y,Yana I,Ohno H,Seiki M||The Journal of cell biology (155:1345)||2001|
|Not Applicable||Not Cited||Distinct subpopulations of sensory afferents require F11 or axonin-1 for growth to their target layers within the spinal cord of the chick.||Perrin FE,Rathjen FG,Stoeckli ET||Neuron (30:707)||2001|
|Not Applicable||Not Cited||Apical localization of wingless transcripts is required for wingless signaling.||Simmonds AJ,dosSantos G,Livne-Bar I,Krause HM||Cell (105:197)||2001|
|Not Applicable||Not Cited||An olfactory sensory map in the fly brain.||Vosshall LB,Wong AM,Axel R||Cell (102:147)||2000|
|Not Applicable||Not Cited||CCR5 provides a signal for microbial induced production of IL-12 by CD8 alpha+ dendritic cells.||Aliberti J,Reis e Sousa C,Schito M,Hieny S,Wells T,Huffnagle GB,Sher A||Nature immunology (1:83)||2000|
|Not Applicable||Not Cited||Neurogenesis in the neocortex of adult primates.||Gould E,Reeves AJ,Graziano MS,Gross CG||Science (New York, N.Y.) (286:548)||1999|