|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Donkey / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 350|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against rabbit, rat, mouse and human IgG|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||20 µg/ml||
Intracerebral transplantation of interleukin 13-producing mesenchymal stem cells limits microgliosis, oligodendrocyte loss and demyelination in the cuprizone mouse model.
A-21081 was used in immunohistochemistry - frozen section to develop an in vivo approach using intracerebral grafting of mesenchymal stem cells to secrete interleukin 13
|Le Blon D,Guglielmetti C,Hoornaert C,Quarta A,Daans J,Dooley D,Lemmens E,Praet J,De Vocht N,Reekmans K,Santermans E,Hens N,Goossens H,Verhoye M,Van der Linden A,Berneman Z,Hendrix S,Ponsaerts P||Journal of neuroinflammation (13:null)||2016|
Smooth muscle cell transplantation improves bladder contractile function in streptozocin-induced diabetic rats.
A-21081 was used in immunohistochemistry - frozen section to discuss factors that contribute to defects observed in diabetic bladders
|Gopinath C,Ponsaerts P,Fransen E,Boeykens N,Pauwels P,Wyndaele JJ||Cytotherapy (15:869)||2013|
|Not Applicable||Not Cited||
Extracellular matrix-induced transforming growth factor-beta receptor signaling dynamics.
A-21081 was used in immunocytochemistry to elucidate TGF-beta receptor dynamics and consequential signaling
|Garamszegi N,Garamszegi SP,Samavarchi-Tehrani P,Walford E,Schneiderbauer MM,Wrana JL,Scully SP||Oncogene (29:2368)||2010|
|Not Applicable||Not Cited||Identification of an intracellular trafficking and assembly pathway for HIV-1 gag.||Perlman M,Resh MD||Traffic (Copenhagen, Denmark) (7:731)||2006|
|Not Applicable||Not Cited||SPARC regulates extracellular matrix organization through its modulation of integrin-linked kinase activity.||Barker TH,Baneyx G,Cardó-Vila M,Workman GA,Weaver M,Menon PM,Dedhar S,Rempel SA,Arap W,Pasqualini R,Vogel V,Sage EH||The Journal of biological chemistry (280:36483)||2005|
|Not Applicable||Not Cited||Olfactory horizontal basal cells demonstrate a conserved multipotent progenitor phenotype.||Carter LA,MacDonald JL,Roskams AJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (24:5670)||2004|