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|Tested species reactivity||Goat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Chicken / IgY|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human, mouse and rabbit IgG prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This Chicken anti-goat antibody reacts with IgG heavy chains and all classes of immunoglobulin light chains from goat. Chicken secondary antibodies have gained popularity because they demonstrate a lower level of nonspecific binding. Chicken antibodies lack a classic “Fc” domain and will not bind to protein A or protein G, nor will they bind to mammalian IgG Fc receptors.
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatocellular carcinoma originates from hepatocytes and not from the progenitor/biliary compartment.
A-21468 was used in immunohistochemistry - paraffin section to elucidate the origin of hepatocellular carcinoma.
|Mu X,Español-Suñer R,Mederacke I,Affò S,Manco R,Sempoux C,Lemaigre FP,Adili A,Yuan D,Weber A,Unger K,Heikenwälder M,Leclercq IA,Schwabe RF||The Journal of clinical investigation (125:3891)||2015|
|Not Applicable||Not Cited||The role of dystroglycan in PDGF-BB-dependent migration of activated hepatic stellate cells/myofibroblasts.||Kastanis GJ,Hernandez-Nazara Z,Nieto N,Rincón-Sanchez AR,Popratiloff A,Dominguez-Rosales JA,Lechuga CG,Rojkind M||American journal of physiology. Gastrointestinal and liver physiology (301:G464)||2011|
|Not Applicable||Not Cited||The ClC-3 chloride transport protein traffics through the plasma membrane via interaction of an N-terminal dileucine cluster with clathrin.||Zhao Z,Li X,Hao J,Winston JH,Weinman SA||The Journal of biological chemistry (282:29022)||2007|
|Not Applicable||Not Cited||Suppression of beta-amyloid precursor protein signaling into the nucleus by estrogens mediated through complex formation between the estrogen receptor and Fe65.||Bao J,Cao C,Zhang X,Jiang F,Nicosia SV,Bai W||Molecular and cellular biology (27:1321)||2007|
|Not Applicable||Not Cited||Aberrant expression of ID2, a suppressor of B-cell-specific gene expression, in Hodgkin's lymphoma.||Renné C,Martin-Subero JI,Eickernjäger M,Hansmann ML,Küppers R,Siebert R,Bräuninger A||The American journal of pathology (169:655)||2006|