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Western blot detection of Goat IgG in 50ng per lane of (lane 1) Goat IgG and a control with no antibody (lane 2) using a Goat IgG polyclonal antibody (Product # PA1-28659) at a dilution of 1:1000 following incubation overnight at 4°C. Secondary detection was performed using a Peroxidase donkey secondary antibody at 1:40,000 for 30 min at room temperature.
|Tested species reactivity||Goat|
|Host / Isotype||Donkey / IgG|
|Immunogen||Full length native Goat IgG (purified).|
|Storage buffer||TBS, pH 8.0, with 10mg/ml BSA, 50% glycerol, 0.1mM ZnCl2, 1mM MgCl2|
|Contains||0.01% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent|
|Western Blot (WB)||Assay-dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28659 detects Goat IgG from goat samples.
PA1-28659 has been successfully used in immunohistochemistry (frozen tissue) and immunohistochemistry (paraffin tissue) applications.
The PA1-28659 immunogen is: Full length native Goat IgG (purified).
Thermo Scientific Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.