Invitrogen

Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488

Product Image Gallery

Chicken IgY (H+L) Secondary Antibody in Immunofluorescence (IF) — Mouse intestine cryosection showing basement membranes labeled with our chicken IgY anti-fibronectin antibody (Product # A21316) and the Alexa Fluor® 488 Goat Anti-Chicken IgG (Product # A-11039, green). Goblet cells and crypt cells were labeled with Alexa Fluor® 594 wheat germ agglutinin (Product # W11262, red). The microvillar brush border and smooth muscle layers were visualized with Alexa Fluor® 680 phalloidin (Product # A22286, pseudocolored purple). The section was counterstained with DAPI (Product # D1306, D3571, D21490, blue).

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1-10 µg/mL

Immunocytochemistry (ICC)

1-10 µg/mL

Immunofluorescence (IF)

1-10 µg/mL

Immunohistochemistry (IHC)

1-10 µg/mL

Published Applications

Immunohistochemistry (IHC)

See 10 publications below

Flow Cytometry (Flow)

See 2 publications below

Immunocytochemistry (ICC)

See 7 publications below

Immunohistochemistry (Paraffin) (IHC (P))

See 4 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 4 publications below

Immunohistochemistry - Free Floating (IHC (Free))

See 3 publications below

Miscellaneous PubMed (MISC)

See 15 publications below

Product Specifications

Species Reactivity

Chicken

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Immunogen

Gamma Immunoglobins Heavy and Light chains

Conjugate

Alexa Fluor® 488

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

2 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.5

Contains

5mM sodium azide

Storage conditions

4° C, store in dark

RRID

AB_2534096

Target

IgY

Antibody Form

Whole Antibody

Product Specific Information

To minimize cross-reactivity, these goat anti-chicken IgY whole antibodies have been affinity-purified. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.

Target Information

We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.

Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.

Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488

Product Details

Product Specific Information

Target Information

Material safety data sheets (MSDS)

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