Goat anti-Chicken IgY (H+L) Secondary Antibody, DSB-X Biotin conjugate for IF
This product (Catalog Number D-20701) has been discontinued. Please contact Customer Service for possible alternatives.
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doesn't perform in your experiment as described on our website
or datasheet, we'll replace the product at no cost to you, or
if you prefer, we'll provide you with a credit for a future
purchase.*
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the
product for use in their own experiment using appropriate negative and positive controls.
The DSB-X biotin goat anti-chicken IgG is prepared from antibodies that have not been cross-adsorbed. DSB-X biotin is a derivative of desthiobiotin, a stable biotin percursuor. DSB-X biotin utilizes a seven-atom spacer to increase the available of the DSB-Xbiotin conjugate to bind in the deep biotin-binding pocket of streptpavidin or avidin. Whereas harsh chaotropic agents and low pH are required to dissociate a biotin complex from avidin or steptavidin, binding can be reversed by adding excess D-desthiobiotin or natural D-biotin at neutral pH and at room temperature.
Background/Target Information
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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