|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 633|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against mouse, rabbit and bovine serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 5 publications below|
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Cross-comparison of protein recognition of sialic acid diversity on two novel sialoglycan microarrays.||Padler-Karavani V,Song X,Yu H,Hurtado-Ziola N,Huang S,Muthana S,Chokhawala HA,Cheng J,Verhagen A,Langereis MA,Kleene R,Schachner M,de Groot RJ,Lasanajak Y,Matsuda H,Schwab R,Chen X,Smith DF,Cummings RD,Varki A||The Journal of biological chemistry (287:22593)||2012|
|Not Applicable||Not Cited||Loss of Kitlow progenitors, reduced stem cell factor and high oxidative stress underlie gastric dysfunction in progeric mice.||Izbeki F,Asuzu DT,Lorincz A,Bardsley MR,Popko LN,Choi KM,Young DL,Hayashi Y,Linden DR,Kuro-o M,Farrugia G,Ordog T||The Journal of physiology (588:3101)||2010|
|Not Applicable||Not Cited||CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry.||Harris HJ,Farquhar MJ,Mee CJ,Davis C,Reynolds GM,Jennings A,Hu K,Yuan F,Deng H,Hubscher SG,Han JH,Balfe P,McKeating JA||Journal of virology (82:5007)||2008|
|Not Applicable||Not Cited||Enhanced fluorescence cell imaging with metal-coated slides.||Moal EL,Fort E,Lévêque-Fort S,Cordelières FP,Fontaine-Aupart MP,Ricolleau C||Biophysical journal (92:2150)||2007|
|Not Applicable||Not Cited||The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis.||Suh CI,Stull ND,Li XJ,Tian W,Price MO,Grinstein S,Yaffe MB,Atkinson S,Dinauer MC||The Journal of experimental medicine (203:1915)||2006|