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Mouse IgG Fc Secondary Antibody (SA5-10374-AFP800) in ICC/IF
Immunofluorescence analysis of Goat anti-Mouse IgG Fc, Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 800 (Product # SA5-10374-AFP800) was performed on fixed and permeabilized HeLa cells. Cells were blocked with 2% BSA for 1 hour at room temperature and probed with a cocktail of primary antibodies against beta-Tubulin (Product # 32-2600, 1:500) and HMGB1 (Product # MA5-17278, 1:200) overnigh... View More
Product Details
SA5-10374-AFP800
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
1:500
Immunocytochemistry (ICC/IF)
1:1,000
Peptide Array (Array)
1:1,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Purified Mouse IgG
Conjugate
Excitation/Emission Max
789/794 nm
View spectra
Form
Liquid
Concentration
1.0 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008 Bromonitrodioxane, 0.008 Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
RRID
Target
IgG Fc
Antibody Form
Whole Antibody
Product Specific Information
Goat anti-Mouse IgG Fc, Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 800
specifically detects the Fc portion of mouse IgG1, mouse IgG2a, and mouse IgG2b with no reactivity to Mouse IgG F(ab)2 fragment and with minimum cross reactivity to Human IgG, Rat IgG and Hamster IgG.
The sensitivity and specificity of each lot of Goat anti-Mouse IgG Fc, Superclonal™ Recombinant Secondary Antibody is confirmed using ELISA and then taken up for conjugation.
Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency.
Alexa Fluor™ Plus conjugated recombinant antibodies provide bright signals and high signal-to-noise for your critical experiments.
It is recommended that the antibody is carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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