|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 635|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
We also provide a version of this antibody that has been cross-adsorbed against additional species (A-31575).
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Structure binding relationship of human surfactant protein D and various lipopolysaccharide inner core structures.
A-31574 was used to study the relationship between lipopolysaccharide inner core structures and the structure binding relationship of human surfactant protein D
|Reinhardt A,Wehle M,Geissner A,Crouch EC,Kang Y,Yang Y,Anish C,Santer M,Seeberger PH||Journal of structural biology (195:387)||2016|
|Not Applicable||Not Cited||
Neurons and Glial Cells Are Added to the Female Rat Anteroventral Periventricular Nucleus During Puberty.
A-31574 was used in immunohistochemistry - free floating to examine sex-specific development of the rat anteroventral periventricular nucleus
|Mohr MA,Garcia FL,DonCarlos LL,Sisk CL||Endocrinology (157:2393)||2016|
Neurobehavioral, cellular, and molecular consequences of single and multiple mild blast exposure.
A-31574 was used in ELISA to assess the effect of blast frequency on multiple-injured rats
|Kamnaksh A,Kwon SK,Kovesdi E,Ahmed F,Barry ES,Grunberg NE,Long J,Agoston D||Electrophoresis (33:3680)||2012|
Factors affecting blast traumatic brain injury.
A-31574 was used in reverse phase protein array to report that exposure to blast acoustics increases anxiety and triggers behavioral and molecular changes even without injury
|Kamnaksh A,Kovesdi E,Kwon SK,Wingo D,Ahmed F,Grunberg NE,Long J,Agoston DV||Journal of neurotrauma (28:2145)||2011|