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FluoCells prepared slide #2 (Prod # F14781), which shows bovine pulmonary artery endothelial cells (BPAEC) that have been stained with an anti-β-tubulin mouse monoclonal antibody in conjunction with BODIPY FL goat anti-mouse IgG (Prod # B2752) for labeling microtubules, Texas Red-X phalloidin (Prod # T7471) for labeling F-actin and DAPI (Prod # D1306, D3571, D21490) for labeling nuclei. This multiple-exposure image was acquired using bandpass optical filter sets appropriate for DAPI, fluorescein and Texas Red dye.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5, with 1.5% BSA|
|Storage Conditions||-20° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Storage and reconstitution: reconstitute using 0.5 mL deionized water. The product can be stored up to 2 weeks at 2-8°C. For longer storage, divide into single-use aliquots and freeze at or below -20°C. Frozen aliquots are stable for at least 6 months.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.