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Mouse fibroblast microtubules labeled with a mouse monoclonal anti–ß-tubulin antibody in conjunction with biotin-XX goat anti–mouse IgG antibody (Cat. no. B2763) and then developed for visualization with alkaline phosphatase–mediated techniques using the ELF® 97 Cytological Labeling Kit (Cat. no. E6603). This kit's novel ELF® 97 phosphatase substrate yields a yellow-green–fluorescent precipitate at the site of alkaline phosphatase activity. Prior to antibody labeling, mouse fibroblasts were fixed and permeabilized in the presence of cytoskeletal stabilizing buffer and treated with paclitaxel (Cat. no. P3456) to stabilize microtubule structures. The image was deconvolved using Huygens software (Scientific Volume Imaging, http://www.svi.nl/).
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.