Alexa Fluor® 488 (Cat. No. A11017) or fluorescein (Cat. No. F11021) conjugates of goat anti–mouse IgG antibody F(ab and quote;)2 fragment were used to detect HEp-2 cells probed with human anti-nuclear antibodies. Samples were continuously illuminated and images were collected every 5 sec with a cooled CCD camera. Normalized intensity data demonstrate the difference in photobleaching rates.
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and serum|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Regulation of macrophage phagocytosis of apoptotic cells by cAMP.||Rossi AG,McCutcheon JC,Roy N,Chilvers ER,Haslett C,Dransfield I||Journal of immunology (Baltimore, Md. : 1950) (160:3562)||1998|
|Not Applicable||Not Cited||Reducing cellular autofluorescence in flow cytometry: an in situ method.||Mosiman VL,Patterson BK,Canterero L,Goolsby CL||Cytometry (30:151)||1997|