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The spinal cord, dorsal root ganglia and sympathetic ganglia from the brachial region of a chick cryosection at stage 23 (Hamburger and Hamilton numbering) were identified with the mouse monoclonal anti-human neuronal protein HuC/HuD antibody (Cat. no. A21271) and visualized with fluorescein-labeled goat anti-mouse IgG antibody (Cat. no. F2761). The primary antibody recognizes an antigen localized to neuronal cell bodies and is ideal for identifying and counting neurons in both early and late development. Human, mouse, avian and zebrafish tissues have all demonstrated similar neuron-specific labeling patterns. Image contributed by Michael Marusich, Monoclonal Antibody Facility, University of Oregon, Eugene, Oregon.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1-10 µg/mL|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.