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Actin labeled with BODIPY FL phallacidin (B607) and vinculin, a cytoskeletal focal adhesion protein, tagged with a monoclonal anti-vinculin antibody that was subsequently probed with Texas Red goat anti–mouse IgG antibody (T862). The large triangular cell is a fibroblast containing green actin stress fibers terminating in red focal adhesions. The neighboring polygonal cell, a rat neonatal cardiomyocyte, contains green striated actin in the myofibrils terminating in the focal adhesions. The close apposition of the two stains results in a yellowish-orange color. Image contributed by Mark B. Snuggs and W. Barry VanWinkle, University of Texas, Houston.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG and human serum prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.