Representative images demonstrating lot-to-lot consistency of performance across three lots of Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (A28174) in immunocytochemistry. No primary control to assess background is shown. Endogenous Cytochrome-C (A) and endogenous Nucleostemin (B) in U20S human osteocarcinoma cells was labeled (green) with A) Mouse anti-Cytochrome C and B) Mouse anti-Nucleostemin primary antibody, Goat anti-mouse IgG (H+L) Superclonal™ Secondary Antibody, Unconjugated (A28174) (0.4 ug/ml, 1:2500) and visualized using Alexa Fluor® 488 Rabbit anti-Goat IgG (H+L) as tertiary antibody (A11078) (1:400). Nuclei (blue) were stained with SlowFade® Gold Antifade Mountant using DAPI (S36938) (1:50). F-actin (red) was stained with Alexa Fluor® 594 Phalloidin (A12381) (1:200).
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Recombinant full-length protein|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.01-0.5 µg/ml|
|Immunocytochemistry (ICC)||0.1-2 µg/ml|
|Immunofluorescence (IF)||0.1-2 µg/ml|
|Western Blot (WB)||0.05-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The sensitivity and specificity of each lot is confirmed using ELISA.
Minimal cross-reactivity with rabbit, rat, human, bovine, guinea pig and donkey IgG is observed.
Superclonal™ secondary antibodies are developed to be highly specific for both heavy and light chains of IgG. Utilizing the power of recombinant technology and multiple phenotypic screens, Superclonal™ antibodies are selected for enhanced specificity to the target species IgG. These recombinant secondary antibodies show similar performance as traditional polyclonal secondary antibodies in common immunoassay applications, with minimal cross-reactivity to other commonly-used species. In addition, their recombinant animal-free-origin helps ensures lot-to-lot consistency, reducing the need to optimize each lot in relevant assays.
Superclonal™ secondary antibodies are designed to allow scientists to achieve the accurate results they need, without some of the concerns that can impact traditional polyclonal secondary antibodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.