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Western blot analysis of CREB was performed by loading 25ug of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB (Product # MA1-083) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product #34077).
|Tested species reactivity||Mouse|
|Published species reactivity||Eubacteria , Mouse , Human|
|Host / Isotype||Goat / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with proprietary stabilizer|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:60-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
This antibody may cross-react with immunoglobulins from other species.
Working dilutions for Western blotting using SuperSignal West Substrates:
SuperSignal West Pico Substrate - 1:250-1:1250
SuperSignal West Dura Substrate - 1:600-1:3000
SuperSignal West Femto Substrate - 1:1250-1:6000
ECL Western Blotting Substrate - 1:20-1:125
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Class D ?-lactamases: are they all carbapenemases?
32430 was used in western blot to study the carbapenemase activity of class D beta-lactamases
|Antunes NT,Lamoureaux TL,Toth M,Stewart NK,Frase H,Vakulenko SB||Antimicrobial agents and chemotherapy (58:2119)||2014|
Regulation of the transcriptional activation of the androgen receptor by the UXT-binding protein VHL.
32430 was used in western blot to study the mechanism by which the UXT-binding protein VHL modulates androgen receptor transcriptional activation
|Chen S,Chen K,Zhang Q,Cheng H,Zhou R||The Biochemical journal (456:55)||2013|
The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity.
32430 was used in western blot to study the mechanism by which Ubc9-mediated SUMOylation of ZHX1 modulates its stability and functional activity
|Chen S,Yu X,Lei Q,Ma L,Guo D||Journal of cellular biochemistry (114:2323)||2013|
Post-ischemic estradiol treatment reduced glial response and triggers distinct cortical and hippocampal signaling in a rat model of cerebral ischemia.
32430 was used in western blot to study the effects on cortical and hippocampal signaling and glial responses of post ischemic estradiol treatment using a rat model of cerebral ischemia
|P?rez-?lvarez MJ,Maza Mdel C,Anton M,Ordo?ez L,Wandosell F||Journal of neuroinflammation (9:null)||2012|