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Spindle microtubules labeled with an anti-tubulin primary antibody followed by (left) goat anti–mouse IgG antibody colloidal gold or (right) NANOGOLD Fab' fragment of goat anti–mouse IgG antibody (Cat. no. N24915). Micrographs (magnification = 1300x) were generated by D. Vandre and R. Burry, Ohio State University. Image courtesy of Nanoprobes, Inc.
|Tested species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.4, with 0.1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Immunomicroscopy (IM)||0.2-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The NANOGOLD Fab' fragment of goat anti-mouse IgG is covalently conjugated with uniform, 1.4 nm-diameter gold clusters. When used with our LI Silver Enhancement Kit (L-24919), the NANOGOLD conjugates can be used as a probe in immunoblotting, light microscopy or electron microscopy.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.