|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||IgG gamma 1|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against mouse IgM, IgA, IgG2a, IgG2b and IgG3, pooled human sera and purified human paraproteins|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Video-rate confocal microscopy for single-molecule imaging in live cells and superresolution fluorescence imaging.
A10538 was used in immunocytochemistry to develop a microscope for single-molecule imaging in live cells and superresolution fluorescence imaging to study cAMP receptors of Dictyostelium discoideum
|Lee J,Miyanaga Y,Ueda M,Hohng S||Biophysical journal (103:1691)||2012|