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Invitrogen
Goat anti-Mouse IgG2a Superclonal™ Secondary Antibody, PE
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FIGURE: 1 / 2
Mouse IgG2a Secondary Antibody (SA5-10370-PE) in Flow
HeLa cells were fixed using IC Fixation buffer (Product # 00-8222-49), Fc block was performed using Fc Receptor Binding Inhibitor Polyclonal Antibody, eBioscience™ (Product # 14-9161-73). Cells were permeabilized using permeabilization buffer (Product # 00-8333-56) and processed using the same permeabilization buffer and stained without (blue histogram) or with PDI Monoclonal Antibody (RL90) (Product # MA3-... View More
Product Details
SA5-10370-PE
Applications
Tested Dilution
Flow Cytometry (Flow)
0.5 µg/test
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Secondary Antibody
Immunogen
Purified Mouse IgG
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
5mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Target
IgG2a
Antibody Form
Whole Antibody
Product Specific Information
This Goat anti-Mouse IgG2a, Superclonal™ Recombinant Secondary Antibody, PE is specific to mouse IgG2a with minimum cross reactivity towards Hamster IgG.
Superclonal™ recombinant secondary antibodies are produced using a multifaceted clonal selection process that involves several phenotypic screens to ensure enhanced specificity, animal origin free formulation and lot-to-lot consistency. .
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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