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Invitrogen
F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 647
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FIGURE: 1 / 3
Mouse IgG2b Secondary Antibody (SA5-10382-AFP647) in ICC/IF
Immunofluorescence analysis of F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 647 (Product # SA5-10382-AFP647) was performed on fixed and permeabilized HeLa cells blocked with SuperBlock™ Blocking Buffer (Product # 37515) at room temperature and stained with Lamin A/C Monoclonal Antibody (mab636) (Product # MA3-1000, 1:200) in SuperBlock™ Blocking Buffer (Product # 37515, 1:20... View More
Product Details
SA5-10382-AFP647
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
1:500
Immunocytochemistry (ICC/IF)
1:1,000
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG, F(ab)
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
27H27L7
Immunogen
Purified Mouse IgG
Conjugate
Excitation/Emission Max
658/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
proprietary buffer, pH 6.3-7.3
Contains
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Wet ice
Target
IgG2b
Antibody Form
F(ab) Fragment
Product Specific Information
F(ab)-Goat anti-Mouse IgG2b Recombinant Secondary Antibody, Alexa Fluor™ Plus 647 is designed to specifically detect the mouse IgG2b isotype showing no detectable reactivity with immunoglobulins from any other tested species.
This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency.
F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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