|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse Mu immunonglobin|
|Conjugate||Alexa Fluor® 546|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgGl, IgG2a, IgG2b, IgG3, IgA, human serum and purified human paraproteins prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Querkopf is a key marker of self-renewal and multipotency of adult neural stem cells.
A-21045 was used in immunohistochemistry to elucidate the factors regulating adult neural stem cells in vivo
|Sheikh BN,Dixon MP,Thomas T,Voss AK||Journal of cell science (125:295)||2012|
Growth of primary embryo cells in a microculture system.
A-21045 was used in immunocytochemistry to report the optimal perfusion conditions for the growth of primary mouse embryonic fibroblasts and embryonic stem cells using a microfluidic perfusion culture system
|Villa M,Pope S,Conover J,Fan TH||Biomedical microdevices (12:253)||2010|
|Not Applicable||Not Cited||Differential dendritic Ca2+ signalling in young and mature hippocampal granule cells.||Stocca G,Schmidt-Hieber C,Bischofberger J||The Journal of physiology (586:3795)||2008|
|Not Applicable||Not Cited||Induction of pluripotent stem cells from adult human fibroblasts by defined factors.||Takahashi K,Tanabe K,Ohnuki M,Narita M,Ichisaka T,Tomoda K,Yamanaka S||Cell (131:861)||2007|