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Bovine pulmonary artery endothelial cells (BPAEC) incubated with the fixable, mitochondrion-selective MitoTracker® Red CMXRos (M7512). After staining, the cells were formaldehyde-fixed, acetone-permeabilized, treated with DNase-free RNase and counterstained using SYTOX® Green nucleic acid stain (S7020). Microtubules were labeled with a mouse monoclonal anti–ß-tubulin antibody, biotin-XX goat anti–mouse IgG antibody (B2763) and Cascade Blue® NeutrAvidin biotin-binding protein (A2663). This photograph was taken using multiple exposures through bandpass optical filters appropriate for Texas Red® dye, fluorescein and DAPI using a Nikon Labophot 2 microscope equipped with a Quadfluor epi-illumination system.
|Tested species reactivity||Rabbit|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Cross Adsorption||Against human IgG, human serum, mouse IgG and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/mL|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.