|Tested species reactivity||Rabbit|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Storage buffer||PBS, pH 7.5, with 0.1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, store in dark|
|Antibody Form||F(ab')2 Fragment|
|Tested Applications||Dilution *|
|Immunomicroscopy (IM)||0.2-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 2 publications below|
Alexa Fluor 488 FluoroNanogold Fab' fragment of goat anti-rabbit IgG is covalently conjugated with uniform, 1.4 nm-diameter gold clusters and our exceptionally bright, photostable Alexa Fluor 488 dye (which is spectrally similar to fluorescein). FluoroNanogold conjugates can be used as probes in fluorescence microscopy and, when used with our LI Silver Enhancement Kit (Cat. no. L-24919), in immunoblotting, light microscopy or electron microscopy.
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Epidermal growth factor receptor is involved in enterocyte anoikis through the dismantling of E-cadherin-mediated junctions.||Lugo-Martínez VH,Petit CS,Fouquet S,Le Beyec J,Chambaz J,Pinçon-Raymond M,Cardot P,Thenet S||American journal of physiology. Gastrointestinal and liver physiology (296:G235)||2009|
|Not Applicable||Not Cited||The keratin-binding protein Albatross regulates polarization of epithelial cells.||Sugimoto M,Inoko A,Shiromizu T,Nakayama M,Zou P,Yonemura S,Hayashi Y,Izawa I,Sasoh M,Uji Y,Kaibuchi K,Kiyono T,Inagaki M||The Journal of cell biology (183:19)||2008|