|Immunofluorescence (IF)||1-10 µg/mL|
|Western Blot (WB)||1:2,000-1:20,000|
|Immunohistochemistry (IHC)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage conditions||4° C, store in dark|
|Cross Adsorption||Against human serum and mouse IgG and serum|
|Antibody Form||Whole Antibody|
DSB-X biotin is a derivative of desthiobiotin, a stable biotin percursuor. DSB-X biotin utilizes a seven-atom spacer to increase the available of the DSB-X biotin conjugate to bind in the deep biotin-binding pocket of streptpavidin or avidin. Whereas harsh chaotropic agents and low pH are required to dissociate a biotin complex from avidin or steptavidin, binding can be reversed by adding excess D-desthiobiotin or natural D-biotin at neutral pH and at room temperature.
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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