Mitotic spindles isolated from sea urchin eggs that are labeled with YOYO®-1 iodide (Cat. no. Y3601) and a monoclonal anti–ß-tubulin antibody in conjunction with Texas Red® goat anti–rat IgG antibody (Cat. no. T6392). This image was generated by epifluorescence microscopy. Image contributed by John Murray, University of Pennsylvania.
|Tested species reactivity||Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against human IgG, human serum, mouse IgG, mouse serum and bovine serum|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 8 publications below|
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Directed cell growth on protein-functionalized hydrogel surfaces.||Hynd MR,Frampton JP,Dowell-Mesfin N,Turner JN,Shain W||Journal of neuroscience methods (162:255)||2007|
|Not Applicable||Not Cited||Role of bone marrow cells in the healing process of mouse experimental glomerulonephritis.||Hayakawa M,Ishizaki M,Hayakawa J,Migita M,Murakami M,Shimada T,Fukunaga Y||Pediatric research (58:323)||2005|
|Not Applicable||Not Cited||Multiple effects on Clostridium perfringens binding, uptake and trafficking to lysosomes by inhibitors of macrophage phagocytosis receptors.||O'Brien DK,Melville SB||Microbiology (Reading, England) (149:1377)||2003|
|Not Applicable||Not Cited||Inducible dimerization of FGFR1: development of a mouse model to analyze progressive transformation of the mammary gland.||Welm BE,Freeman KW,Chen M,Contreras A,Spencer DM,Rosen JM||The Journal of cell biology (157:703)||2002|
|Not Applicable||Not Cited||Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes.||Dermine JF,Duclos S,Garin J,St-Louis F,Rea S,Parton RG,Desjardins M||The Journal of biological chemistry (276:18507)||2001|
|Not Applicable||Not Cited||Angiogenesis monitored by perfusion with a space-filling microbead suspension.||Springer ML,Ip TK,Blau HM||Molecular therapy : the journal of the American Society of Gene Therapy (1:82)||2000|
|Not Applicable||Not Cited||VEGF gene delivery to muscle: potential role for vasculogenesis in adults.||Springer ML,Chen AS,Kraft PE,Bednarski M,Blau HM||Molecular cell (2:549)||1998|
|Not Applicable||Not Cited||The fate of self-reactive B cells depends primarily on the degree of antigen receptor engagement and availability of T cell help.||Fulcher DA,Lyons AB,Korn SL,Cook MC,Koleda C,Parish C,Fazekas de St Groth B,Basten A||The Journal of experimental medicine (183:2313)||1996|