Invitrogen

Golgi protein 58k Polyclonal Antibody

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Protocols

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Golgi protein 58k Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Golgi protein 58k in Human Liver using a Golgi protein 58k monoclonal antibody (Product #PA1-9000) at 3.8 µg/mL. The Human Liver tissue section was paraffin embeded and detected using steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Golgi protein 58k Antibody in Western Blot (WB) — Western blot was performed using Anti-Golgi protein 58k Polyclonal Antibody (Product # PA1-9000) and a 58kDa band corresponding to Golgi protein 58k was observed in Hep G2, Mouse Liver and Rat Liver but was not observed in HeLa, A549, HEK-293, Mouse Kidney, Mouse Heart and Rat Brain which are reported to be negative. Whole Cell Extract-WCL (30 µg lysate) of Hep G2 (Lane 1), HeLa (Lane 2), A549 (Lane 3), HEK-293 (Lane 4), Mouse Liver (Lane 5), Mouse Kidney (Lane 6), Mouse Heart (Lane 7), Rat Liver (Lane 8) and Rat Brain (Lane 9), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.1ug/ml) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Golgi protein 58k Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Golgi protein 58k in HepG2 cells using a polyclonal antibody (Product #PA1-9000). HepG2 cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.

Golgi protein 58k Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of Golgi protein 58k was observed in Hep G2, Mouse Liver and Rat Liver and was not observed in HeLa, A549, HEK-293, Mouse Kidney, Mouse Heart and Rat Brain using Anti-Golgi protein 58k Polyclonal Antibody (Product # PA1-9000) in Western Blot. {RE}

Product Details

PA1-9000

Applications	Tested Dilution	Publications
Western Blot (WB)	0.03-0.1 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	3-5 µg/mL	-
Flow Cytometry (Flow)	10 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Pig
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Internal sequence corresponding to amino acids CLREQGRGKDQPGRL.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2278649

Product Specific Information

This antibody is predicted to react with mouse, porcine and rat based on sequence homology.

Target Information

Golgi Protein, 58K is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Formimidoyltetrahydrofolate cyclodeaminase; Formimidoyltransferase-cyclodeaminase; Formiminotetrahydrofolate cyclodeaminase; formiminotransferase cyclodeaminase; Formiminotransferase-cyclodeaminase; FTCD; Glutamate formimidoyltransferase; Glutamate formiminotransferase; Glutamate formyltransferase; LCHC1

Gene Aliases: FTCD; LCHC1

UniProt ID: (Pig) P53603, (Human) O95954, (Mouse) Q91XD4

Entrez Gene ID: (Pig) 397517, (Human) 10841, (Mouse) 14317

Function(s)

histidine metabolic process histidine catabolic process cytoskeleton organization metabolic process cellular metabolic process histidine catabolic process to glutamate and formamide histidine catabolic process to glutamate and formate tetrahydrofolate interconversion folic acid-containing compound metabolic process

Process(es)

folic acid binding glutamate formimidoyltransferase activity formimidoyltetrahydrofolate cyclodeaminase activity protein binding microtubule binding formimidoyltransferase activity catalytic activity transferase activity lyase activity