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HeLa cells labeled with anti–golgin-97 antibody and detected using Alexa Fluor® 488 Goat Anti–Mouse IgG antibody. Fixed, permeabilized HeLa cells were labeled with anti–golgin-97 monoclonal antibody (A21270) and detected using Alexa Fluor® 488 Goat Anti–Mouse IgG antibody (A11001). The cells were counterstained with DAPI (D1306, D3571, D21490). (Cat. No. A21270).
|Tested species reactivity||Human|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1.0 ug/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer.
A-21270 was used in western blot to describe a method for the quantification and characterization of exosomes and microvesicles.
|Pospichalova V,Svoboda J,Dave Z,Kotrbova A,Kaiser K,Klemova D,Ilkovics L,Hampl A,Crha I,Jandakova E,Minar L,Weinberger V,Bryja V||Journal of extracellular vesicles (4:null)||2015|
An optimized isolation of biotinylated cell surface proteins reveals novel players in cancer metastasis.
A-21270 was used in western blot to investigate the existence of novel proteins in cancer metastasis.
|Karhemo PR,Ravela S,Laakso M,Ritamo I,Tatti O,Mäkinen S,Goodison S,Stenman UH,Hölttä E,Hautaniemi S,Valmu L,Lehti K,Laakkonen P||Journal of proteomics (77:87)||2012|
Chemical structure requirements and cellular targeting of microRNA-122 by peptide nucleic acids anti-miRs.
A-21270 was used in western blot to characterize microRNA oligonucleotide inhibitors.
|Torres AG,Fabani MM,Vigorito E,Williams D,Al-Obaidi N,Wojciechowski F,Hudson RH,Seitz O,Gait MJ||Nucleic acids research (40:2152)||2012|
A method to separate nuclear, cytosolic, and membrane-associated signaling molecules in cultured cells.
A-21270 was used in western blot to describe a method to separate the cytosolic, nucleosolic, nuclear insoluble, and membrane components of various mammalian cell lines.
|Liu X,Fagotto F||Science signaling (4:null)||2011|
Mre11 is expressed in mammalian mitochondria where it binds to mitochondrial DNA.
A-21270 was used in western blot to study the role of Mre in the upkeep of mitochondrial DNA.
|Dmitrieva NI,Malide D,Burg MB||American journal of physiology. Regulatory, integrative and comparative physiology (301:R632)||2011|
HSP47 and FKBP65 cooperate in the synthesis of type I procollagen.
A-21270 was used in immunocytochemistry to examine the cooperation of HSP47 and FKBP65 and its relation to osteogenesis imperfecta
|Duran I,Nevarez L,Sarukhanov A,Wu S,Lee K,Krejci P,Weis M,Eyre D,Krakow D,Cohn DH||Human molecular genetics (24:1918)||2015|
RC/BTB2 is essential for formation of primary cilia in mammalian cells.
A-21270 was used in immunocytochemistry to assess if RC/BTB2 contributes to ciliogenesis
|Zhang L,Li W,Ni J,Wu J,Liu J,Zhang Z,Zhang Y,Li H,Shi Y,Teves ME,Song S,Strauss JF,Zhang Z||Cytoskeleton (Hoboken, N.J.) (72:171)||2015|
Nile Tilapia Neu3 sialidases: molecular cloning, functional characterization and expression in Oreochromis niloticus.
A-21270 was used in immunocytochemistry to elucidate the role of fish Neu3 sialidases
|Chigwechokha PK,Komatsu M,Itakura T,Shiozaki K||Gene (552:155)||2014|
Characterization of Rabaptin-5 γ isoform.
A-21270 was used in immunocytochemistry to characterize the rabaptin-5gamma isoform
|Korobko EV,Kiselev SL,Korobko IV||Biochemistry. Biokhimii¿a¿ (79:856)||2014|
Intracellular itinerary of internalised β-secretase, BACE1, and its potential impact on β-amyloid peptide biogenesis.
A-21270 was used in immunocytochemistry to examine the intracellular trafficking of β-Secretase.
|Chia PZ,Toh WH,Sharples R,Gasnereau I,Hill AF,Gleeson PA||Traffic (Copenhagen, Denmark) (14:997)||2013|
Alternative transcripts and 3'UTR elements govern the incorporation of selenocysteine into selenoprotein S.
A-21270 was used in immunocytochemistry to report mechanisms that regulate SelS expression.
|Bubenik JL,Miniard AC,Driscoll DM||PloS one (8:null)||2013|
Regulation of WASH-dependent actin polymerization and protein trafficking by ubiquitination.
A-21270 was used in immunocytochemistry to report the K63-linked ubiquitination regulation of MAGE-L2-TRIM27-mediated retrograde transport.
|Hao YH,Doyle JM,Ramanathan S,Gomez TS,Jia D,Xu M,Chen ZJ,Billadeau DD,Rosen MK,Potts PR||Cell (152:1051)||2013|
A novel domain regulating degradation of the glomerular slit diaphragm protein podocin in cell culture systems.
A-21270 was used in immunocytochemistry to study the slit diaphragm protein podocin.
|Gödel M,Ostendorf BN,Baumer J,Weber K,Huber TB||PloS one (8:null)||2013|
Amyloid precursor protein (APP) traffics from the cell surface via endosomes for amyloid β (Aβ) production in the trans-Golgi network.
A-21270 was used in immunocytochemistry to investigate the intracellular transport of amyloid precursor protein
|Choy RW,Cheng Z,Schekman R||Proceedings of the National Academy of Sciences of the United States of America (109:E2077)||2012|
GM130 gain-of-function induces cell pathology in a model of lysosomal storage disease.
A-21270 was used in immunocytochemistry to investigate the role of GM30 during lysosomal biogenesis.
|Roy E,Bruyère J,Flamant P,Bigou S,Ausseil J,Vitry S,Heard JM||Human molecular genetics (21:1481)||2012|
Splice variant-specific cellular function of the formin INF2 in maintenance of Golgi architecture.
A-21270 was used in immunocytochemistry to determine the localization pattern and cellular function of the CAAX isoforms.
|Ramabhadran V,Korobova F,Rahme GJ,Higgs HN||Molecular biology of the cell (22:4822)||2011|
Down-regulation of oestrogen receptor-β associates with transcriptional co-regulator PATZ1 delocalization in human testicular seminomas.
A-21270 was used in immunocytochemistry to study ERβ interactions with PATZ in the testes.
|Esposito F,Boscia F,Franco R,Tornincasa M,Fusco A,Kitazawa S,Looijenga LH,Chieffi P||The Journal of pathology (224:110)||2011|
The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex.
A-21270 was used in immunocytochemistry to study the function of cloned human SAC.
|Rohde HM,Cheong FY,Konrad G,Paiha K,Mayinger P,Boehmelt G||The Journal of biological chemistry (278:52689)||2003|
Golgin subfamily A member 1, gap junction protein, alpha 4, 37kD, golgi autoantigen, golgin subfamily a, 1, golgin-97, gap junction protein, alpha 4, 37kD, golgi autoantigen, golgin subfamily a, 1