Anti-human golgin-97 antibodies were originally isolated from the serum of a patient with the autoimmune disease known as Sjögren's syndrome. These antibodies recognize a 97 kDa protein called golgin-97, a member of the granin family of proteins and a peripheral membrane protein localized on the cytoplasmic face of the Golgi apparatus. Because the antibody recognizes a protein unique to the Golgi apparatus of most vertebrate species, it is useful for immunodetection and identification of the Golgi apparatus in cells.
To prepare a stock solution, reconstitute the antibody in 0.5-1 mL of phosphate-buffered saline, pH 7.4, containing 1% BSA. Store the solution for up to two weeks at 4°C with the addition of 2 mM sodium azide. For longer storage, divide the solution into single-use aliquots and freeze at -20°C.
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. The Golgins are a family of proteins, of which the protein encoded by this gene is a member, that are localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.
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Protein Aliases: gap junction protein, alpha 4, 37kD; golgi autoantigen, golgin subfamily a, 1; Golgin subfamily A member 1; Golgin-97
Gene Aliases: 0710001G09Rik; 2210418B03Rik; AW107649; GOLGA1; Golgi97; golgin-97