Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of Granulins in HEK293 cells using a Granulins recombinant rabbit oligoclonal antibody (Product # 710191) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and a composite image showing actin stained using Alexa Fluor 594 phalloidin (red). Image D is a composite image showing membrane localization.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 573–588 of human PC cell-derived growth factor|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-3 ug/ml|
|Immunofluorescence (IF)||1-3 ug/ml|
|Western Blot (WB)||2-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with equine, mouse, rabbit and rat based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PCDGF is a glycoprotein and growth factor involved in the regulation of mitosis, early embryonic development, cell division, cell migration, cell survival, and hypothalamic differentiation. PCDGF is also known as progranulin because it is the precursor for smaller proteins known as epithelins or granulins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
acrogranin; GEP; GP88; granulin-epithelin; Granulins; PC cell-derived growth factor; PCDGF; PEPI; PGRN; Proepithelin; progranulin
CLN11; GEP; GP88; GRN; PCDGF; PEPI; PGRN