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Western blot analysis of Granzyme B in whole cell extracts from Jurkat using a Granzyme B recombinant rabbit monoclonal antibody (Product # 701395) at a dilution of 1ug/ml. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody followed by chemiluminescence (ECL). Results show a band at ~32kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 26–38 of human Granzyme B|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/ml X 10^6 cells|
|Western Blot (WB)||1-2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Granzyme B is a serine protease contained within cytoplasmic granules produced by cytotoxic T lymphocytes and natural killer cells. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. It can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzyme B has also been shown to play an important role in activation induced cell death of T helper 2 cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C11; cathepsin G-like 1; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA-1; CTLA1; CTSGL1; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin 2; fragmentin-2; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); GrB; HLP; human lymphocyte protein; T-cell serine protease 1-3E
CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; GRB; GZMB; HLP; SECT