|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human granzyme B.|
|Storage buffer||tissue culture supernatant with 1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay dependent.|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody cannot be used on frozen sections in IHC applications.
The Lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is a serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Histopathologic-based prognostic factors of colorectal cancers are associated with the state of the local immune reaction.
MA1-35461 was used in immunohistochemistry to compare the prognostic value of immune criteria versus the AJCC/UICC-TNM staging system in colorectal cancer
|Mlecnik B,Tosolini M,Kirilovsky A,Berger A,Bindea G,Meatchi T,Bruneval P,Trajanoski Z,Fridman WH,Pagès F,Galon J||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (29:610)||2011|
Biomolecular network reconstruction identifies T-cell homing factors associated with survival in colorectal cancer.
MA1-35461 was used in immunohistochemistry to use biomolecular network reconstruction to investigate the immune responses in colorectal cancer patients
|Mlecnik B,Tosolini M,Charoentong P,Kirilovsky A,Bindea G,Berger A,Camus M,Gillard M,Bruneval P,Fridman WH,Pagès F,Trajanoski Z,Galon J||Gastroenterology (138:1429)||2010|
|Not Applicable||Not Cited||
Localization and identification of granzymes A and B-expressing cells in normal human lymphoid tissue and peripheral blood.
MA1-35461 was used in immunohistochemistry - paraffin section to measure granzyme A and B expression in non-pathological lymphoid tissue and peripheral blood lymphocyte subpopulations
|Kummer JA,Kamp AM,Tadema TM,Vos W,Meijer CJ,Hack CE||Clinical and experimental immunology (100:164)||1995|