|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal region of human guanylyl cyclase beta 1, soluble.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain tissue.
Soluble guanylyl cyclase catalyses in a Mg2+ or Mn2+ dependent manner, the conversion of guanosine-5 and quote;-triphosphate (GTP) to cyclic guanosine-3 and quote;,5-monophosphate (cGMP) and pyrophosphate. This enzyme is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.