|Tested species reactivity||Guinea pig|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Immunogen||Gamma Immunoglobins Heavy and Light chains|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.5|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Cross Adsorption||Against bovine, chicken, goat, hamster, human, mouse, rabbit, rat and sheep sera prior to conjugation|
|Antibody Form||Whole Antibody|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1-10 µg/ml|
|Immunofluorescence (IF)||1-10 µg/mL|
|Immunohistochemistry (IHC)||1-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
To minimize cross-reactivity, these goat anti-guinea pig IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against bovine, chicken, goat, hamster, human, mouse, rabbit, rat, and sheep sera prior to conjugation. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there are may be the presence of endogenous immunoglobulins.
Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 488 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.
We offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.
Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.
Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Regulating the dorsal neural tube expression of Ptf1a through a distal 3' enhancer.
A-11073 was used in immunohistochemistry - frozen section to assess dorsalneural tube expression of Ptf1a regulated through a distal 3' enhancer
|Mona B,Avila JM,Meredith DM,Kollipara RK,Johnson JE||Developmental biology (418:216)||2016|
|Not Applicable||Not Cited||Pseudosynapsis and decreased stringency of meiotic repair pathway choice on the hemizygous sex chromosome of Caenorhabditis elegans males.||Checchi PM,Lawrence KS,Van MV,Larson BJ,Engebrecht J||Genetics (197:543)||2014|
|Not Applicable||Not Cited||Lysosomal membrane permeability stimulates protein aggregate formation in neurons of a lysosomal disease.||Micsenyi MC,Sikora J,Stephney G,Dobrenis K,Walkley SU||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:10815)||2013|
|Not Applicable||Not Cited||Inactivation of plasma membrane-localized CDPK-RELATED KINASE5 decelerates PIN2 exocytosis and root gravitropic response in Arabidopsis.||Rigó G,Ayaydin F,Tietz O,Zsigmond L,Kovács H,Páy A,Salchert K,Darula Z,Medzihradszky KF,Szabados L,Palme K,Koncz C,Cséplo A||The Plant cell (25:1592)||2013|
|Not Applicable||Not Cited||ATP-gated ion channels mediate adaptation to elevated sound levels.||Housley GD,Morton-Jones R,Vlajkovic SM,Telang RS,Paramananthasivam V,Tadros SF,Wong AC,Froud KE,Cederholm JM,Sivakumaran Y,Snguanwongchai P,Khakh BS,Cockayne DA,Thorne PR,Ryan AF||Proceedings of the National Academy of Sciences of the United States of America (110:7494)||2013|
|Not Applicable||Not Cited||Microsomal triacylglycerol transfer protein (MTP) is required to expand tracheal lumen in Drosophila in a cell-autonomous manner.||Baer MM,Palm W,Eaton S,Leptin M,Affolter M||Journal of cell science (125:6038)||2012|
|Not Applicable||Not Cited||Synaptic refinement of an inhibitory topographic map in the auditory brainstem requires functional Cav1.3 calcium channels.||Hirtz JJ,Braun N,Griesemer D,Hannes C,Janz K,Löhrke S,Müller B,Friauf E||The Journal of neuroscience : the official journal of the Society for Neuroscience (32:14602)||2012|
|Not Applicable||Not Cited||Deletion of G¿Z protein protects against diet-induced glucose intolerance via expansion of ß-cell mass.||Kimple ME,Moss JB,Brar HK,Rosa TC,Truchan NA,Pasker RL,Newgard CB,Casey PJ||The Journal of biological chemistry (287:20344)||2012|
|Not Applicable||Not Cited||Purified human pancreatic duct cell culture conditions defined by serum-free high-content growth factor screening.||Hoesli CA,Johnson JD,Piret JM||PloS one (7:null)||2012|
|Not Applicable||Not Cited||METT-10, a putative methyltransferase, inhibits germ cell proliferative fate in Caenorhabditis elegans.||Dorsett M,Westlund B,Schedl T||Genetics (183:233)||2009|
|Not Applicable||Not Cited||Functional interactions between Mldp (LSDP5) and Abhd5 in the control of intracellular lipid accumulation.||Granneman JG,Moore HP,Mottillo EP,Zhu Z||The Journal of biological chemistry (284:3049)||2009|
|Not Applicable||Not Cited||Targeting green fluorescent protein to dendritic membrane in central neurons.||Kameda H,Furuta T,Matsuda W,Ohira K,Nakamura K,Hioki H,Kaneko T||Neuroscience research (61:79)||2008|
|Not Applicable||Not Cited||Impaired insulin secretion and glucose intolerance in synaptotagmin-7 null mutant mice.||Gustavsson N,Lao Y,Maximov A,Chuang JC,Kostromina E,Repa JJ,Li C,Radda GK,Südhof TC,Han W||Proceedings of the National Academy of Sciences of the United States of America (105:3992)||2008|
|Not Applicable||Not Cited||Dynamics of embryonic pancreas development using real-time imaging.||Puri S,Hebrok M||Developmental biology (306:82)||2007|
|Not Applicable||Not Cited||Specialized inhibitory synaptic actions between nearby neocortical pyramidal neurons.||Ren M,Yoshimura Y,Takada N,Horibe S,Komatsu Y||Science (New York, N.Y.) (316:758)||2007|
|Not Applicable||Not Cited||GATA factor translation is the final downstream step in the amino acid/target-of-rapamycin-mediated vitellogenin gene expression in the anautogenous mosquito Aedes aegypti.||Park JH,Attardo GM,Hansen IA,Raikhel AS||The Journal of biological chemistry (281:11167)||2006|
|Not Applicable||Not Cited||A monovalent streptavidin with a single femtomolar biotin binding site.||Howarth M,Chinnapen DJ,Gerrow K,Dorrestein PC,Grandy MR,Kelleher NL,El-Husseini A,Ting AY||Nature methods (3:267)||2006|
|Not Applicable||Not Cited||Pancreatic beta-cells secrete insulin in fast- and slow-release forms.||Michael DJ,Ritzel RA,Haataja L,Chow RH||Diabetes (55:600)||2006|
|Not Applicable||Not Cited||Selective vulnerability of dopaminergic neurons to microtubule depolymerization.||Ren Y,Liu W,Jiang H,Jiang Q,Feng J||The Journal of biological chemistry (280:34105)||2005|
|Not Applicable||Not Cited||LXRbeta is required for adipocyte growth, glucose homeostasis, and beta cell function.||Gerin I,Dolinsky VW,Shackman JG,Kennedy RT,Chiang SH,Burant CF,Steffensen KR,Gustafsson JA,MacDougald OA||The Journal of biological chemistry (280:23024)||2005|
|Not Applicable||Not Cited||Stem cell division is regulated by the microRNA pathway.||Hatfield SD,Shcherbata HR,Fischer KA,Nakahara K,Carthew RW,Ruohola-Baker H||Nature (435:974)||2005|
|Not Applicable||Not Cited||Protein phosphatase-1 binding to scd5p is important for regulation of actin organization and endocytosis in yeast.||Chang JS,Henry K,Wolf BL,Geli M,Lemmon SK||The Journal of biological chemistry (277:48002)||2002|
|Not Applicable||Not Cited||Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics.||Ono S,Ono K||The Journal of cell biology (156:1065)||2002|
|Not Applicable||Not Cited||Bonus, a Drosophila homolog of TIF1 proteins, interacts with nuclear receptors and can inhibit betaFTZ-F1-dependent transcription.||Beckstead R,Ortiz JA,Sanchez C,Prokopenko SN,Chambon P,Losson R,Bellen HJ||Molecular cell (7:753)||2001|
|Not Applicable||Not Cited||The metabotropic GABAB receptor directly interacts with the activating transcription factor 4.||Nehring RB,Horikawa HP,El Far O,Kneussel M,Brandstätter JH,Stamm S,Wischmeyer E,Betz H,Karschin A||The Journal of biological chemistry (275:35185)||2000|