Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Inter-species cross-reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiSerum has not been adsorbed for such cross-reactivity. Consequently it is not species-specific.
In immunoelectrophoresis use 2 µL serum or equivalent against 120 µL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 µL antiserum in 3 mm diameter center well and 2 µL serum samples (neat and serially diluted in 2 mm diameter peripheral wells. Different bleedings of the immunized animals are pooled to obtain a broad spectrum balanced against the varying concentrations of the individual serum protein components.
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Guinea Pig Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Guinea Pig serum antibody is suitable for use in veterinary research and biotechnology applications and is ideal for investigators in Cancer and Cell Biology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.