|Tested species reactivity||Tag|
|Published species reactivity||Virus, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||9 amino acid peptide to influenza virus hemagglutinin.|
|Storage buffer||TBS, pH 8.2, with 50% glycerol|
|Contains||3mM sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein on western blot or to immunoprecipitate the recombinant protein when doing binding studies with transfected cells. Balb/C mice were immunized with a 9 aa peptide to influenza virus hemagglutinin (HA). The resultant hybridomas were selected for reactivity with the immunogen by ELISA and with the antigen by western blot. The antibody was purified to >95% homogeneity by standard chromatographic techniques.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites.
MA1-12429 was used in western blot to suggest that secretory vesicles deliver RhoGAP Rgd1p to the bud tip and bud neck.
|Lefèbvre F,Prouzet-Mauléon V,Hugues M,Crouzet M,Vieillemard A,McCusker D,Thoraval D,Doignon F||Eukaryotic cell (11:590)||2012|
Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization.
MA1-12429 was used in western blot to elucidate how Dma1 and Dma2 regulate Swe1
|Raspelli E,Cassani C,Lucchini G,Fraschini R||Molecular biology of the cell (22:2185)||2011|
Oxidation state governs structural transitions in peroxiredoxin II that correlate with cell cycle arrest and recovery.
MA1-12429 was used in western blot to investigate the structural transitions in peroxiredoxin II regulated by oxidation state and its correlation with cell cycle arrest and recovery
|Phalen TJ,Weirather K,Deming PB,Anathy V,Howe AK,van der Vliet A,Jönsson TJ,Poole LB,Heintz NH||The Journal of cell biology (175:779)||2006|
Negative regulation of TSC1-TSC2 by mammalian D-type cyclins.
MA1-12429 was used in western blot to investigate the influence of cyclin D1 on tumor suppressor TSC1 and 2 function
|Zacharek SJ,Xiong Y,Shumway SD||Cancer research (65:11354)||2005|
Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha.
MA1-12429 was used in immunoprecipitation to study the role of the DNA replication factor Mcm10 in regulating DNA polymerase alpha stability and chromatin association
|Ricke RM,Bielinsky AK||Molecular cell (16:173)||2004|
Transduction of interleukin-2 antiapoptotic and proliferative signals via Akt protein kinase.
MA1-12429 was used in immunocytochemistry to investigate the mechanism for the antiapoptotic and proliferative functions of interleukin 2
|Ahmed NN,Grimes HL,Bellacosa A,Chan TO,Tsichlis PN||Proceedings of the National Academy of Sciences of the United States of America (94:3627)||1997|