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|Tested species reactivity||Tag|
|Published species reactivity||Dog, Nematode, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||A synthetic peptide corresponding to the nine amino acid sequence HA-tag (YPYDVPDYA).|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ZO proteins redundantly regulate the transcription factor DbpA/ZONAB.
32-6700 was used in western blot to study the role of ZO proteins in the regulation of transcription factor DbpA/ZONAB
|Spadaro D,Tapia R,Jond L,Sudol M,Fanning AS,Citi S||The Journal of biological chemistry (289:22500)||2014|
Host acyl coenzyme A binding protein regulates replication complex assembly and activity of a positive-strand RNA virus.
32-6700 was used in western blot to investigate how vesicular spherules and stacks of appressed double-membrane layers are formed.
|Zhang J,Diaz A,Mao L,Ahlquist P,Wang X||Journal of virology (86:5110)||2012|
Intersection of the multivesicular body pathway and lipid homeostasis in RNA replication by a positive-strand RNA virus.
32-6700 was used in western blot to investigate the relationship between lipid synthesis and brome mosaic virus RNA replication.
|Wang X,Diaz A,Hao L,Gancarz B,den Boon JA,Ahlquist P||Journal of virology (85:5494)||2011|
|Not Applicable||Not Cited||
C-terminal COOH of integrin β1 is necessary for β1 association with the kindlin-2 adapter protein.
32-6700 was used in ELISA, immunoprecipitation, and western blot to study the role of C-terminal COOH of integrin beta1 in its association with kindlin-2 adapter protein
|Fitzpatrick P,Shattil SJ,Ablooglu AJ||The Journal of biological chemistry (289:11183)||2014|
|Not Applicable||Not Cited||
The Yersinia virulence effector YopM binds caspase-1 to arrest inflammasome assembly and processing.
32-6700 was used in immunocytochemistry to investigate the important role of YopM in Yersinia pestis infection.
|LaRock CN,Cookson BT||Cell host & microbe (12:799)||2012|
|Nematode||1:500||Isoforms of the polarity protein par6 have distinct functions.||Gao L,Macara IG||The Journal of biological chemistry (279:41557)||2004|
|Human||Not Cited||Cbl-dependent ubiquitination is required for progression of EGF receptors into clathrin-coated pits.||Stang E,Blystad FD,Kazazic M,Bertelsen V,Brodahl T,Raiborg C,Stenmark H,Madshus IH||Molecular biology of the cell (15:3591)||2004|