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|Tested species reactivity||Tag|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||HA-tag conjugated to KLH.|
|Storage buffer||TBS, pH 7.4, with 10mg/ml BSA|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:5000 - 1:15000|
|Western Blot (WB)||1:1000 -1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA1-29751 detects HA from rabbit samples.
PA1-29751 has been successfully used in Western blot and ELISA applications.
The PA1-29751 immunogen is from rabbits that were immunized with HA-tag conjugated to KLH. After multiple immunizations in Freunds adjuvant, serum was collected. Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support then conjugated with HRP (horseradish Peroxidase).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Kruppel-like factor 15 is critical for vascular inflammation.
PA1-29751 was used in immunoprecipitation to study the mechanism by which Kruppel-like factor-15 modulates the inflammatory activation of vascular smooth muscle cells
|Lu Y,Zhang L,Liao X,Sangwung P,Prosdocimo DA,Zhou G,Votruba AR,Brian L,Han YJ,Gao H,Wang Y,Shimizu K,Weinert-Stein K,Khrestian M,Simon DI,Freedman NJ,Jain MK||The Journal of clinical investigation (123:4232)||2013|
HA; HA Epitope Tag; HA-tag; human influenza hemagglutinin