|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Fusion protein from rat HAP1.|
|Storage buffer||PBS with 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 µg/10^6 cells|
|Immunohistochemistry (IHC)||0.5-1.0 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||0.5-1.0 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.
Suggested positive control: rat brain.
Huntington's disease is an autosomal dominant neurodegerative disorder caused by an expanded polyglutamine repeat region in the huntingtin gene. HAP1 is a huntingtin associated protein that shows neuronal localization and moves with huntingtin in nerve fibers. The ability of HAP1 to bind to huntingtin is enhanced by the expanded polyglutamine repeat region typical of the mutant form of the protein associated with Huntington's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Huntingtin associated protein 1 regulates trafficking of the amyloid precursor protein and modulates amyloid beta levels in neurons.
MA1-46412 was used in immunohistochemistry, immunoprecipitation, and western blot to study the regulation of APP trafficing and neuronal beta-amyloid levels by Huntingtin-associated protein-1
|Yang GZ,Yang M,Lim Y,Lu JJ,Wang TH,Qi JG,Zhong JH,Zhou XF||Journal of neurochemistry (122:1010)||2012|