Western blot of HAUSP in HeLa nuclear extract (Lane 1) HeLa (Lane 2) A431 (Lane 3) MCF7 (Lane 4) and NIH-3T3 (Lane 5) and blocked by pre-incubation with the immunizing peptide (lanes 6 - 10) using the same lysates using a HAUSP polyclonal antibody (Product # PA1-31831) at a dilution of 1:500 and detected using a 1:5000 dilution of HRP Goat-anti-Rabbit IgG at an Exposure time of 30 seconds.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide TDDPPRITQNPVC, corresponding to amino acids 30-41 of Human HAUSP.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31831 detects HAUSP from human samples.
PA1-31831 has been successfully used in Western blot applications.
The PA1-31831 immunogen is: Synthetic peptide: TDDPPRITQNPVC, corresponding to amino acids 30-41 of Human HAUSP.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.