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Immunofluorescent analysis of HeLa cells using a HBO1/MYST2 polyclonal antibody (Product # PA5-11255). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a HBO1/MYST2 polyclonal antibody (Product # PA5-11255) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 213-246 amino acids from the central region of human HBO1/MYST2|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse and rat based on sequence homology.
HBO1/MYST2 is a histone acetyltransferase which specifically represses AR-mediated transcription. It may play a role in DNA replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
EC 126.96.36.199; HBO1; HBOa; histone acetyltransferase binding to ORC1; Histone acetyltransferase MYST2; K(lysine) acetyltransferase 7; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2; MYST histone acetyltransferase 2; MYST protein 2; MYST-2; MYST2
HBO1; HBOA; KAT7; MYST2; ZC2HC7